Journal: iScience

Article Title: Chronic HDM exposure shows time-of-day and sex-based differences in inflammatory response associated with lung circadian clock disruption

doi: 10.1016/j.isci.2023.107580

Figure Lengend Snippet: Serum levels of total and HDM-specific immunoglobulins show a time-of-day response and sex-based differences to chronic HDM exposure Total IgE, Total IgG, HDM-specific IgE, HDM-specific IgG, HDM-specific IgG1, HDM-specific IgG2b, HDM-specific IgM, and HDM-specific IgA in the serum of chronic PBS- and HDM-exposed females and males at ZT0 and ZT12 were determined by ELISA. Data were expressed as ng/ml and mg/ml for total IgE and total IgG, respectively. HDM-specific immunoglobulins (IgE, IgG, IgG1, IgG2b, IgM, and IgA) in the serum were determined by commercially available ELISA kits (Chondrex, Inc.). Unable to detect HDM-specific IgG2a and IgG3 levels in PBS- and HDM-exposed mice. Data for the HDM-specific immunoglobulins were expressed as absorbance at 450 nm or μg/ml. Data are shown as mean ± SEM, Two-way ANOVA followed by Tukey’s multiple comparison test (n = 5/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, compared to respective control (PBS) at ZT0 or ZT12; # p < 0.05, compared to HDM at ZT0 vs. ZT12; # # p < 0.01, compared to HDM at ZT0 vs. ZT12. Summary statistics for interaction between Treatment x Sex x Time were analyzed using generalized linear modeling using R (see Table S2 ).

Article Snippet: Mouse Anti-House Dust Mite (HDM) IgG2b Antibody Assay Kit , Chondrex, Inc. , Cat# 3035.

Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Control

Journal: iScience

Article Title: Chronic HDM exposure shows time-of-day and sex-based differences in inflammatory response associated with lung circadian clock disruption

doi: 10.1016/j.isci.2023.107580

Figure Lengend Snippet:

Article Snippet: Mouse Anti-House Dust Mite (HDM) IgG2b Antibody Assay Kit , Chondrex, Inc. , Cat# 3035.

Techniques: Purification, Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Software

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: A novel bispecific antibody targeting CD3 and prolactin receptor (PRLR) against PRLR-expression breast cancer

doi: 10.1186/s13046-020-01564-4

Figure Lengend Snippet: PRLR-DbsAb stimulates T cell infiltration and the PD-L1 expression unregulated in tumor tissue. sc Tumor cells plus sc effector cells (E/T 1:4) model: ( a ) HE staining of tumor tissue strapped from mice treated with 3 mg/kg PRLR-DbsAb. The above image showed normal tumor tissue, and the following image showed degenerate necrotic tumor tissue; ( b ) Immunohistochemical staining of CD8 in tumor tissue from the mice of different group; ( c ) Immunohistochemical staining of PD-L1 in tumor tissue from the mice of different group. Sc tumor cells plus ip effector cells (1:1) model: ( d ) HE staining; ( e ) Immunofluorescent staining of CD8. Three independent experiments were conducted and representative data is shown in this figure

Article Snippet: Breast cancer cell lines (MDA-MB-231, MCF-7, SKBR-3, and T47D) were incubated with control isotype IgG, PE-conjugated mouse anti-Human PRLR (Sino Biological) or APC-conjugated mouse anti-Human PD-L1 (Sino Biological) on ice for 30 min and then washed twice with FACS buffer (PBS with 2% FBS).

Techniques: Expressing, Staining, Immunohistochemical staining

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: A novel bispecific antibody targeting CD3 and prolactin receptor (PRLR) against PRLR-expression breast cancer

doi: 10.1186/s13046-020-01564-4

Figure Lengend Snippet: PD-1 inhibition increases PRLR-DbsAb mediated cytotoxicity of PRLR expression target cell. FACS histograms of PDL1 expression on different breast cancer cell lines including MDA-MB-231 ( a ) and T47D ( e ). The gray histogram is the fluorescent signal of non-specific IgG control antibody. Freshly isolated PBMCs and the target cells of MDA-MB-231 ( b ) or T47D ( f ) were incubated with or without addition of PD-1 mAb in different concentrations of PRLR-DbsAb at the ratio of 10:1 (E: T) for 20 hours. MDA-MB-231 ( c ) and T47D ( g ) cells were treated with 100 ng/ml PRLR-DbsAb at different time points (3, 10 and 20h) with or without adding PD-1 mAb and LDH release was detected. The expression of PD-L1 ( d and h ) on the corresponding effector cells and target cells is measured by Flow cytometer, respectively. ( i ) FACS histogram of the PD-L1 expression of target T47D with or without addition of 100 ng/ml PRLR mAb. ( j ) PD-L1 expression on CD4+ and CD8+ T cells. PBMCs and target T47D cells were incubated with 100 ng/ml PRLR-DbsAb at the ratio of 10:1 (E: T) for 20 hours. Three independent experiments were performed and the data was represented as the Mean ±SEM. *, ** and *** respectively indicate a statistically significant difference of at least P <0.05, <0.01 and <0.001

Article Snippet: Breast cancer cell lines (MDA-MB-231, MCF-7, SKBR-3, and T47D) were incubated with control isotype IgG, PE-conjugated mouse anti-Human PRLR (Sino Biological) or APC-conjugated mouse anti-Human PD-L1 (Sino Biological) on ice for 30 min and then washed twice with FACS buffer (PBS with 2% FBS).

Techniques: Inhibition, Expressing, Isolation, Incubation, Flow Cytometry

Journal:

Article Title: Co-Purification of Mac-2 Binding Protein with Galectin-3 and Association with Prostasomes in Human Semen

doi: 10.1002/pros.21287

Figure Lengend Snippet: Peptide Matches from Tandem Mass Spectrometry of Proteins Purified by Lactose Affinity Chromatography of Human Prostasomes

Article Snippet: Mouse monoclonal antibodies against human CD26 were from Lab Vision (Fremont, CA).

Techniques: Mass Spectrometry, Purification, Affinity Chromatography, Sequencing, Binding Assay

Journal:

Article Title: Co-Purification of Mac-2 Binding Protein with Galectin-3 and Association with Prostasomes in Human Semen

doi: 10.1002/pros.21287

Figure Lengend Snippet: Protein-protein interaction network of the identified proteins. Cytoscape was used to display the protein-protein interaction map generated from interaction database data pulled down from the HPRD, BioGRID, IntAct, and NCI/Nature Pathway Interaction databases. White nodes indicate the proteins identified in this study following β-galactoside affinity purification. Gray nodes indicate interacting proteins retrieved from the databases. Connecting lines indicate identified protein interactions. Each protein is shown by its gene symbol or entrez identification number. Galectin-3 (LGALS3), M2BP (LGALS3BP), lactoferrin (LTF), PIP (PIP), CD26 (DPP4), seminogelin I (SEMG1), seminogelin 2 (SEMG2), olfactomedin 4 (OLFM4).

Article Snippet: Mouse monoclonal antibodies against human CD26 were from Lab Vision (Fremont, CA).

Techniques: Generated, Affinity Purification

Journal:

Article Title: Co-Purification of Mac-2 Binding Protein with Galectin-3 and Association with Prostasomes in Human Semen

doi: 10.1002/pros.21287

Figure Lengend Snippet: Prostasome purification by size exclusion chromatography and M2BP immunoblot analysis. The membrane fraction from seminal plasma was subjected to size exclusion column chromatography on Sephacryl S300 and prostasomes were collected in the void volume (fractions 14-19). Electroblots of collected fractions were stained for total protein with Ponceau S and for M2BP (R&D polyclonal antibodies) and CD26 immunoreactivity. Molecular weight markers are indicated in kDa.

Article Snippet: Mouse monoclonal antibodies against human CD26 were from Lab Vision (Fremont, CA).

Techniques: Purification, Size-exclusion Chromatography, Western Blot, Column Chromatography, Staining, Molecular Weight